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Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles
JoVE Journal
Biologie
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JoVE Journal Biologie
Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

Purification and Analysis of Caenorhabditis elegans Extracellular Vesicles

DOI:
10.3791/60596-v

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09:30 min

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March 31, 2020

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, , , ,
1Department of Pathology,University of Washington

Kapitel

  • 00:05Introduction
  • 00:27Large C. elegans Population Calculation
  • 01:31Secretome Generation Preparation
  • 02:25Extracellular Vesicle (EV) Purification
  • 05:14EV Quantification
  • 06:26Data Analysis
  • 07:05Results: Representative C. elegans EV Characterization and Analyses
  • 08:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article presents methods for generating, purifying, and quantifying Caenorhabditis elegans extracellular vesicles.

Tags

C. ElegansExtracellular VesiclesPurificationRNA-seqProteomicsSecretomeS-basal SolutionCentrifugationFiltrationWorm CultivationWorm SuspensionSynchronizationNylon MeshVacuumVortexCarbenicillinCholesterolRotator

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