The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.
Hu, J., Derr, N. D. Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation. J. Vis. Exp. (152), e60369, doi:10.3791/60369 (2019).