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Produzione, cristallizzazione e determinazione della struttura del dominio iKK-binding di NEMO

JoVE Journal
Biochemie

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JoVE Journal Biochemie

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

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Produzione, cristallizzazione e determinazione della struttura del dominio iKK-binding di NEMO

7,272 Views

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13:02 min

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December 28, 2019

DOI:

10.3791/60339-v

DOI:

10.3791/60339-v

•

13:02 min

December 28, 2019

•

1 Views

Adam H. Barczewski, Michael J. Ragusa, Dale F. Mierke, Maria Pellegrini

¹Department of Chemistry,Dartmouth College

Tags

NEMO IKK-binding Domain Protein Production Crystallization Structure Determination Coiled-coil Adapters Protein Refolding Protein Concentration Aggregation Solubility French Press Cell Lysis Urea Protein Purification

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