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Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
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Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
Kapitel
- 00:05Titel
- 01:06Live Imaging of Growing Abdominal Epithelia
- 03:24Generation of Genetic Mosaics to Follow Behaviors of Cell Clones
- 04:23Data Processing and Analyses
- 05:57Results: Long-term Live Imaging and Clonal Analysis
- 07:22Conclusion
This protocol is designed for the imaging and analysis of the dynamics of cell orientation and tissue growth in the Drosophila abdominal epithelia as the fruit fly undergoes metamorphosis. The methodology described here can be applied to the study of different developmental stages, tissues, and subcellular structures in Drosophila or other model organisms.
