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siRNA 电穿孔以在单纯疱疹病毒类型 1-感染单细胞衍生的丹突细胞中调制自噬
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
DOI:

09:10 min

October 28, 2019

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Kapitel

  • 00:04Titel
  • 01:02Generation and Handling of Immature Dendritic Cells and Mature Dendritic Cells
  • 02:02Infection Procedure of DCs with HSV-1 and Interference of HSV-1-induced Autophagic Flux via Spautin-1 or Bafilomycin-A1
  • 04:17Interference of HSV-1-induced Autophagic Flux via Electroporation of iDCs Using FIP200-siRNA
  • 06:21Results: Analyses of Autophagic Flux in HSV-1-infected iDCs and mDCs
  • 08:10Conclusion

Summary

Automatische Übersetzung

在这项研究中,我们提出了基于抑制剂和siRNA的策略,以干扰单纯疱疹病毒类型-1(HSV-1)感染的单细胞衍生树突状细胞中的自噬性通量。

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