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Herpes Simpleks Virüs Tip 1-Enfekte Monosit Türetilmiş Dendritik Hücrelerde Otofaji Modüle siRNA Elektroporasyon
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
DOI:

09:10 min

October 28, 2019

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Kapitel

  • 00:04Titel
  • 01:02Generation and Handling of Immature Dendritic Cells and Mature Dendritic Cells
  • 02:02Infection Procedure of DCs with HSV-1 and Interference of HSV-1-induced Autophagic Flux via Spautin-1 or Bafilomycin-A1
  • 04:17Interference of HSV-1-induced Autophagic Flux via Electroporation of iDCs Using FIP200-siRNA
  • 06:21Results: Analyses of Autophagic Flux in HSV-1-infected iDCs and mDCs
  • 08:10Conclusion

Summary

Automatische Übersetzung

Bu çalışmada, Herpes simpleks virüs tip-1 (HSV-1) enfekte monosit kaynaklı dendritik hücrelerde otofatik akı ile müdahale etmek için inhibitör ve siRNA tabanlı stratejiler sürünmektedir.

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