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Journal
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Entwicklungsbiologie
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小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
Please note that all translations are automatically generated.
Click here for the English version.
小鼠原发性颅神经冠细胞的解剖、培养与分析,用于神经冠细胞脱角和迁移研究
DOI:
10.3791/60051-v
•
09:33 min
•
October 03, 2019
•
Sandra Guadalupe Gonzalez Malagon*
1,2
,
Lisa Dobson*
1,3
,
Anna M Lopez Muñoz
,
Marcus Dawson
,
William Barrell
3
,
Petros Marangos
4
,
Matthias Krause
,
Karen J Liu
1
Centre for Craniofacial and Regenerative Biology
,
King’s College London
,
2
Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research
,
University of Ioannina
,
3
Randall Centre of Cell & Molecular Biophysics
,
King’s College London
,
4
Department of Biological Applications and Technology
,
University of Ioannina
Kapitel
00:04
Titel
01:05
Early Somite Stage Embryo Dissection (Day 1)
03:12
Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
04:36
Neural Crest Single Cell Tracking
05:22
Quantification of Neural Crest Cell Migratory Capacity
06:04
Quantification of Neural Crest Cell Area and Circularity
07:00
Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
08:43
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
该协议描述了从小鼠模型颅神经峰细胞的解剖和培养,主要用于研究细胞迁移。我们描述了使用的实时成像技术,并分析了速度和细胞形状的变化。
Tags
Primary Cranial Neural Crest Cells
Neural Crest Cell Delamination
Neural Crest Cell Migration
Neural Plate
Embryonic Dissection
Neural Crest Development
Mouse Embryo
Cell Culture
Entwicklungsbiologie
Article
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