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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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09:33 min
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October 03, 2019
DOI:
1Centre for Craniofacial and Regenerative Biology,King’s College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research,University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics,King’s College London, 4Department of Biological Applications and Technology,University of Ioannina
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Dieses Protokoll beschreibt die Zerlegung und Kultur von kranialen neuralen Kammzellen aus Mausmodellen, in erster Linie für die Untersuchung der Zellmigration. Wir beschreiben die verwendeten Live-Bildgebungstechniken und die Analyse von Geschwindigkeits- und Zellformänderungen.
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Gonzalez Malagon, S. G., Dobson, L., Muñoz, A. M. L., Dawson, M., Barrell, W., Marangos, P., Krause, M., Liu, K. J. Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration. J. Vis. Exp. (152), e60051, doi:10.3791/60051 (2019).
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