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使用果蝇S2细胞进行细胞分裂的活成像
JoVE Journal
Genetik
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JoVE Journal Genetik
Use of Drosophila S2 Cells for Live Imaging of Cell Division
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使用果蝇S2细胞进行细胞分裂的活成像

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00:06 min

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August 23, 2019

DOI:

10.3791/60049-v

DOI:
10.3791/60049-v

•

00:06 min
August 23, 2019

•

6 Views
, ,
1Department of Biology,University of New Mexico

Summary

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使用荧光标记的蛋白质和延时显微镜可以实时可视化细胞分裂。使用此处介绍的协议，用户可以分析细胞分裂时序动力学、线粒主轴组件以及染色体的组装和分离。RNA干扰（RNAi）介导的基因敲除后这些事件的缺陷可以进行评估和量化。

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Cite this Article

Dewey, E. B., Parra, A. S., Johnston, C. A. Use of Drosophila S2 Cells for Live Imaging of Cell Division. J. Vis. Exp. (150), e60049, doi:10.3791/60049 (2019).

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