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4-티우라실(Ers4tU)을 가진 생체내 RNA의 매우 신속하고 특정한 대사 라벨링
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
Please note that all translations are automatically generated. Click here for the English version.

4-티우라실(Ers4tU)을 가진 생체내 RNA의 매우 신속하고 특정한 대사 라벨링

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00:11 min

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August 22, 2019

DOI:

10.3791/59952-v

DOI:
10.3791/59952-v

•

00:11 min
August 22, 2019

•

8 Views
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1Wellcome Centre for Cell Biology, School of Biological Sciences,University of Edinburgh

Summary

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효모 사카로미세세스 세레비시아에서새로 전사된 RNA를 민감하게 그리고 구체적으로 정화하기 위해 티오레이드 우라실을 사용한다.

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Cite this Article

Barrass, J. D., Beggs, J. D. Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU). J. Vis. Exp. (150), e59952, doi:10.3791/59952 (2019).

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