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Journal / Immunology and Infection / A Luciferase-fluorescent Reporter Influenza Virus …
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Immunology and Infection

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JoVE Journal Immunology and Infection
A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
 

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection

Article DOI: 10.3791/59890-v • 05:21 min • August 14th, 2019
August 14th, 2019 •
1, 2, 1,3, 1
1Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, 2Division of Allergy/Immunology and Rheumatology, Department of Medicine, University of Rochester, 3Center for Animal Health Research, INIA-CISA

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Summary
Automatic Translation

Influenza A viruses (IAVs) are contagious respiratory pathogens that cause annual epidemics and occasional pandemics. Here, we describe a protocol to track viral infections in vivo using a novel recombinant luciferase and fluorescence-expressing bi-reporter IAV (BIRFLU). This approach provides researchers with an excellent tool to study IAV in vivo.

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Luciferase-fluorescent Reporter Influenza Virus Live Imaging Quantification Viral Infection Recombinant Influenza Viruses Fluorescent Protein Reporters Bioluminescent Protein Reporters Bireporter Influenza Virus In Vitro Studies In Vivo Studies Single Recombinant Influenza Virus Luciferase Substrate Mice Handling Bioluminescent Signal Detection Intranasal Inoculation Imaging Software Parameters For Imaging Chest Shaving For Signal Detection
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