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Isolation of Salivary Epithelial Cells from Human Salivary Glands for In Vitro Growth as Salispheres or Monolayers
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Kapitel
- 00:04Titel
- 00:54Tissue Digestion and Well Coating with BMM (Basement Membrane Matrix)
- 02:07Filtering of Undigested Tissue, RBC Lysis, and Cell Plating
- 03:53Subculturing the Salispheres and Maintenance on BMM
- 05:23Subculturing the Salispheres on Treated Plastic Tissue Culture Dishes
- 06:43Results: Primary Human Salisphere Cells Cultured as Spheres or as a Monolayer
- 07:10Conclusion
We present a method for isolating and cultivating primary human salivary gland-derived epithelial cells. These cells exhibit gene expression patterns consistent with them being of salivary epithelial origin and can be grown as salispheres on basement membrane matrices derived from Engelbreth-Holm-Swarm tumor cells or as monolayers on treated culture dishes.
