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Short-Term Free-Floating Slice Cultures from the Adult Human Brain
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Short-Term Free-Floating Slice Cultures from the Adult Human Brain

Short-Term Free-Floating Slice Cultures from the Adult Human Brain

8,367 Views

09:14 min

November 05, 2019

DOI:

09:14 min
November 05, 2019

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Summary

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A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain diseases.

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