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毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析
JoVE Journal
Krebsforschung
This content is Free Access.
JoVE Journal
Krebsforschung
Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
Please note that all translations are automatically generated.
Click here for the English version.
毛细管电泳质谱法提取培养细胞中的水代谢物进行代谢组学分析
DOI:
10.3791/59551-v
•
11:39 min
•
June 09, 2019
•
Ami Maruyama
2
,
Kenjiro Kami
,
Kazunori Sasaki
,
Hajime Sato
,
Yuzo Sato
2
,
Katsuya Tsuchihara
,
Hideki Makinoshima
2,4
1
Shonai Regional Industry Promotion Center
,
2
Tsuruoka Metabolomics Laboratory
,
National Cancer Center
,
3
Human Metabolome Technologies, Inc
,
4
Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center
,
National Cancer Center
Kapitel
00:04
Titel
01:18
Cell Culture on Day 1
04:37
Preparation of Reagents and Pre-washing Centrifugal Filter Units
06:17
Cell Culture on Day 2
07:24
Extraction of Metabolites from Cultured Cells and Ultrafiltration of Cell Extracts
08:55
Results: Diamide Treatment
10:42
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
本文的目的是描述从培养的粘附细胞中提取水代谢物的方案, 用于代谢组学分析, 特别是毛细管电泳质谱法。
Tags
Keyword Extraction:Metabolomics
Capillary Electrophoresis-mass Spectrometry
CEMS
Aqueous Metabolites
Cultured Adherent Cells
Cancer Cells
Metabolite Extraction
Biomarkers
Chemotherapy Response
Cell Culture
Trypsin-EDTA
Cell Suspension
Centrifugation
Article
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