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1Mass Spectrometry Core Facility, School of Life Sciences,Westlake University, 2Department of Biological Sciences,University of Notre Dame, 3Department of Urology, Shanghai East Hospital,Tongji University School of Medicine, 4Boler-Parseghian Center for Rare and Neglected Diseases,University of Notre Dame, 5Harper Cancer Research Institute,University of Notre Dame, 6Tumor Microenvironment and Metastasis Program,Indiana University Melvin and Bren Simon Cancer Center
Kapitel
- 00:04Titel
- 00:32Nitro-Angiotensin II Desalting
- 01:30Primary Amine Alkylation by Dimethyl Labeling
- 02:37Nitrotyrosine to Aminotyrosine Reduction and Biotinylation, Enrichment, and Detection
- 06:03Results: Representative Mass Spectra Analyses
- 06:59Conclusion
O perfilamento Proteomic de proteínas tyrosine-nitrated foi uma técnica desafiante devido à baixa abundância da modificação da 3-nitrotyrosine. Aqui nós descrevemos uma aproximação nova para o enriquecimento e o perfilamento do nitropeptide usando a angiotensina II como o modelo. Este método pode ser estendido para outros sistemas in vitro ou in vivo .
