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HOX Loci Focused CRISPR/sgRNA 라이브러리 심사 식별 중요 CTCF 경계

JoVE Journal
Genetik

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JoVE Journal Genetik

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

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HOX Loci Focused CRISPR/sgRNA 라이브러리 심사 식별 중요 CTCF 경계

8,035 Views

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10:10 min

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March 31, 2019

DOI:

10.3791/59382-v

DOI:

10.3791/59382-v

•

10:10 min

March 31, 2019

•

1 Views

Huacheng Luo, Amin Sobh, Christopher D. Vulpe, Edmond Brewer, Sinisa Dovat, Yi Qiu, Suming Huang

¹Department of Pediatrics,Pennsylvania State University College of Medicine, ²Department of Physiological Sciences,University of Florida, ³Department of Anatomy and Cell Biology,University of Florida

Tags

CRISPR/sgRNA Library CTCF Boundaries HOX Gene Regulation Pooled SgRNA Screening Lentiviral Transduction MOLM13 Cells Puromycin Selection MOI Optimization Noncoding Regulatory Elements

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