Journal
/
Genetik
/
HOX軌跡 Focused CRISPR/sgRNA ライブラリ スクリーニングを識別する重要な CTCF の境界
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
Please note that all translations are automatically generated. Click here for the English version.

HOX軌跡 Focused CRISPR/sgRNA ライブラリ スクリーニングを識別する重要な CTCF の境界

8,035 Views

•

10:10 min

•

March 31, 2019

DOI:

10.3791/59382-v

DOI:
10.3791/59382-v

•

10:10 min
March 31, 2019

•

1 Views
, , , , , ,
1Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Physiological Sciences,University of Florida, 3Department of Anatomy and Cell Biology,University of Florida

Tags

CRISPR/sgRNA LibraryCTCF BoundariesHOX Gene RegulationPooled SgRNA ScreeningLentiviral TransductionMOLM13 CellsPuromycin SelectionMOI OptimizationNoncoding Regulatory Elements

Read Article

Cite this Article

Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y., Huang, S. HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries. J. Vis. Exp. (145), e59382, doi:10.3791/59382 (2019).

Download .ris file

Copy

Share Video

.

Copy