/

/

公司重点调查的社区图书馆筛选确定关键 CTCF 边界

JoVE Journal
Genetik

Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Genetik

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Please note that all translations are automatically generated. Click here for the English version.

公司重点调查的社区图书馆筛选确定关键 CTCF 边界

8,035 Views

•

10:10 min

•

March 31, 2019

DOI:

10.3791/59382-v

DOI:

10.3791/59382-v

•

10:10 min

March 31, 2019

•

1 Views

Huacheng Luo, Amin Sobh, Christopher D. Vulpe, Edmond Brewer, Sinisa Dovat, Yi Qiu, Suming Huang

¹Department of Pediatrics,Pennsylvania State University College of Medicine, ²Department of Physiological Sciences,University of Florida, ³Department of Anatomy and Cell Biology,University of Florida

Tags

CRISPR/sgRNA Library CTCF Boundaries HOX Gene Regulation Pooled SgRNA Screening Lentiviral Transduction MOLM13 Cells Puromycin Selection MOI Optimization Noncoding Regulatory Elements

Read Article