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HOX المكاني مركزة كريسبر/سجرنا "مكتبة الفرز تحديد الحرجة كتكف الحدود"
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JoVE Journal Genetik
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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HOX المكاني مركزة كريسبر/سجرنا "مكتبة الفرز تحديد الحرجة كتكف الحدود"

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10:10 min

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March 31, 2019

DOI:

10.3791/59382-v

DOI:
10.3791/59382-v

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10:10 min
March 31, 2019

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1 Views
, , , , , ,
1Department of Pediatrics,Pennsylvania State University College of Medicine, 2Department of Physiological Sciences,University of Florida, 3Department of Anatomy and Cell Biology,University of Florida

Tags

CRISPR/sgRNA LibraryCTCF BoundariesHOX Gene RegulationPooled SgRNA ScreeningLentiviral TransductionMOLM13 CellsPuromycin SelectionMOI OptimizationNoncoding Regulatory Elements

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Luo, H., Sobh, A., Vulpe, C. D., Brewer, E., Dovat, S., Qiu, Y., Huang, S. HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries. J. Vis. Exp. (145), e59382, doi:10.3791/59382 (2019).

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