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使用多光谱成像流细胞测定法执行体外微核测定的自动化方法
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
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使用多光谱成像流细胞测定法执行体外微核测定的自动化方法

21,788 Views

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12:56 min

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May 13, 2019

DOI:

10.3791/59324-v

DOI:
10.3791/59324-v

•

12:56 min
May 13, 2019

•

14 Views
1Biology Department,Luminex Corporation

Summary

Automatically generated

体外微核测定是评估基因毒性和细胞毒性的成熟方法,但使用人工显微镜对测定进行评分是费力的,具有主观性和分时变异性。本文介绍了为使用多光谱成像流细胞测定执行全自动测试而开发的协议。

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Cite this Article

Rodrigues, M. A. An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry. J. Vis. Exp. (147), e59324, doi:10.3791/59324 (2019).

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