Journal
/
Entwicklungsbiologie
/
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

Dissection of the Drosophila Pupal Retina for Immunohistochemistry, Western Analysis, and RNA Isolation

DOI:
10.3791/59299-v

•

08:47 min

•

March 15, 2019

•

,
1Department of Biology,Wesleyan University

Kapitel

  • 00:04Titel
  • 00:29Eye-Brain Complex Dissection
  • 02:09Tissue Processing for Immunofluorescence
  • 04:56Tissue Processing for Western Blotting
  • 06:07Tissue Processing for RNA Extraction
  • 07:07Results: Representative Pupal Eye Protein and Gene Expression Analyses
  • 08:28Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This paper presents a surgical method for dissecting Drosophila pupal retinas along with protocols for the processing of tissue for immunohistochemistry, western analysis, and RNA-extraction. 

Tags

Keywords: DrosophilaPupal RetinaImmunohistochemistryWestern AnalysisRNA IsolationDissectionEye-brain ComplexMicrodissectionForcepsPBSFixative

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

check_url/de/59299?article_type=v

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
check_url/de/59299?article_type=v

Live-imaging of the Drosophila Pupal Eye
check_url/de/59299?article_type=v

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord
check_url/de/59299?article_type=v

A Rapid and Efficient Method to Dissect Pupal Wings of Drosophila Suitable for Immunodetections or PCR Assays
check_url/de/59299?article_type=v

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
check_url/de/59299?article_type=v

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains
check_url/de/59299?article_type=v

Dissection and Staining of Drosophila Pupal Ovaries
check_url/de/59299?article_type=v

Imaging and Analysis of Tissue Orientation and Growth Dynamics in the Developing Drosophila Epithelia During Pupal Stages
check_url/de/59299?article_type=v

Preparation of Drosophila Larval and Pupal Testes for Analysis of Cell Division in Live, Intact Tissue
check_url/de/59299?article_type=v

Dissection, Immunohistochemistry and Mounting of Larval and Adult Drosophila Brains for Optic Lobe Visualization

Read Article