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Purification of the Dendritic Filopodia-rich Fraction
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JoVE Journal Neurowissenschaften
Purification of the Dendritic Filopodia-rich Fraction

Purification of the Dendritic Filopodia-rich Fraction

5,141 Views

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11:51 min

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May 02, 2019

DOI:

10.3791/59292-v

DOI:
10.3791/59292-v

•

11:51 min
May 02, 2019

•

3 Views
,
1Laboratory for Neurobiology of Synapse,RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit,RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology,RIKEN Center for Brain Science

Summary

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In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

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Cite this Article

Furutani, Y., Yoshihara, Y. Purification of the Dendritic Filopodia-rich Fraction. J. Vis. Exp. (147), e59292, doi:10.3791/59292 (2019).

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