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Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
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Direct Injection of a Lentiviral Vector Highlights Multiple Motor Pathways in the Rat Spinal Cord
Kapitel
- 00:04Titel
- 00:45Surgical Preparation
- 02:13Vertebral Column Exposure
- 03:44Laminectomy
- 04:36Virus Loading
- 05:48Virus Injection and Post-Operative Care
- 06:42Results: Representative Transduction of Neurons in the Spinal Cord and Brainstem
- 07:31Conclusion
This protocol demonstrates injection of a retrogradely transportable viral vector into rat spinal cord tissue. The vector is taken up at the synapse and transported to the cell body of target neurons. This model is suitable for retrograde tracing of important spinal pathways or targeting cells for gene therapy applications.
