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Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
Kapitel
- 00:04Titel
- 00:37Microtubule Preparation
- 02:02Motility Solution
- 03:06Assembling the Flow Cells
- 05:08Flowing the Solutions into a Flow Cell
- 05:45Imaging a Flow Cell
- 06:41Results: Analysis of Assembled Molecular Shuttles
- 07:31Conclusion
We present a protocol to build molecular shuttles, where surface-adhered kinesin motor proteins propel dye-labelled microtubules. Weak interactions of the kinesins with the surface enables their reversible attachment to it. This creates a nanoscale system which exhibits dynamic assembly and disassembly of its components while retaining its functionality.
