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Protein kompleksleri milisaniyelik zaman ölçekte dinamikleri çalışmaya vitro Floresans rezonans enerji transferi içinde kullanma
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Biochemie
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JoVE Journal Biochemie
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
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Protein kompleksleri milisaniyelik zaman ölçekte dinamikleri çalışmaya vitro Floresans rezonans enerji transferi içinde kullanma

7,870 Views

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10:50 min

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March 14, 2019

DOI:

10.3791/59038-v

DOI:
10.3791/59038-v

•

10:50 min
March 14, 2019

•

2 Views
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1Department of Biochemistry,Purdue University, 2Department of Botany and Plant Pathology,Purdue University, 3Center for Plant Biology,Purdue University

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FRETProtein ComplexKineticsAssociationDissociationCOL1CAND17-amino-4-methylcoumarinFlASHCell LysisSonicationCentrifugationGlutathione BeadsElutionThrombinBuffer A

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Garsamo, M., Zhou, Y., Liu, X. Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale. J. Vis. Exp. (145), e59038, doi:10.3791/59038 (2019).

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