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Bir biyogüvenlik seviye 2 ortamda yüksek patojenik Coronaviruses çalışmaya Pseudotyped parçacıklar imalatı
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1Department of Microbiology and Immunology, College of Veterinary Medicine,Cornell University, 2INRA, Virologie et Immunologie Moléculaires, 3Robert Frederick Smith School of Chemical and Biomolecular Engineering,Cornell University, 4Department of Microbiology, College of Agricultural and Life Sciences,Cornell University, 5Horae Gene Therapy Center,University of Massachusetts Medical School
Kapitel
- 00:04Titel
- 00:41Cell Seeding for Pseudotyped Particle Production
- 02:05Three-plasmid Co-transfection
- 03:53Pseudotyped Particles Collection
- 04:46Pseudotyped Particle Infection
- 05:54Infectivity Quantification by Luciferase Assay Readout
- 07:04Results: Infectivity Assays of SARS-CoV S and MERS-CoV S Pseudotyped Particles
- 08:02Conclusion
Burada, pseudotyped parçacıklar spike protein yüksek patojenik virüs Ortadoğu solunum sendromu ve şiddetli akut solunum Sendromu coronaviruses birleştiren bir BSL-2 ayar oluşturmak için bir protokol mevcut. Pseudotyped bu parçacıklar hedef ana bilgisayar hücreler virüs girdiği miktar izin luciferase muhabir gen içerir.
