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A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

A Plate-based Cytotoxicity Assay for the Assessment of Rat Placental Natural Killer Cell Cytolytic Function

DOI:
10.3791/58961-v

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10:44 min

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June 02, 2019

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, , ,
1Department of Emergency Medicine,University of Mississippi Medical Center, 2Department of Experimental Therapeutics and Pharmacology,University of Mississippi Medical Center

Kapitel

  • 00:04Titel
  • 01:28Lymphocyte Cell Isolation from Placentas
  • 02:28Isolation of Natural Killer Cells
  • 06:32Cytotoxicity Assay: Retrieving NK Cells or YAC1 from Culture or Passing Cells
  • 07:51Cytotoxicity Assay: Assay Protocol
  • 09:17Results: Assessment of the Cytotoxicity Assay
  • 09:56Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we provide a detailed methodology to isolate and assess cytotoxic function of natural killer cells from placentas by a colorimetric plate assay. The reduced uterine perfusion pressure rat model of placental ischemia was chosen to demonstrate the antibody-mediated isolation and assessment of the cytotoxic function of natural killer cells.

Tags

– Plate-based Cytotoxicity Assay- Rat Placental Natural Killer Cells- Cytolytic Function- Cytotoxic Activity- Inexpensive Method- Specialized Equipment- Hazardous Materials- CD8+ Cytolytic Cells- Target-effector Cell Ratio Optimization- Mononuclear Cell Isolation- Placenta Homogenization- Density Gradient Centrifugation- Biotin-labeled CD3 Antibody- Magnetic Bead Separation

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