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Journal / Chemistry / 大肠杆菌无细胞蛋白合成:…
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JoVE Journal Chemistry
大肠杆菌无细胞蛋白合成
 
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大肠杆菌无细胞蛋白合成: 一种强大、灵活和可访问的平台技术的协议

Article DOI: 10.3791/58882-v • 09:45 min • February 25th, 2019
February 25th, 2019 •
*1,2, *2,3, 4,5,6,7, 2,3, 2,3
1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University
* These authors contributed equally

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该协议详细介绍了在4天或更短的时间内生成大肠杆菌基细胞提取物并实施体外蛋白质合成反应所需的步骤、成本和设备。为了将这一平台的灵活性用于广泛的应用, 我们讨论了可以调整和优化的反应条件。

Tags

化学 第144期 无细胞蛋白合成 cfps,体外蛋白表达 高通量蛋白合成 tx-tl 合成生物学 无细胞代谢工程 cfme
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