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小鼠表皮角质细胞的分离及其体外克隆培养物
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal Biologie
Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture
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小鼠表皮角质细胞的分离及其体外克隆培养物

15,938 Views

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06:16 min

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August 10, 2019

DOI:

10.3791/58701-v

DOI:
10.3791/58701-v

•

06:16 min
August 10, 2019

•

59 Views
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1Hormel Institute,University of Minnesota

Summary

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该协议的目标是从成年小鼠的背皮中分离表皮角蛋白酶,用于各种下游应用,如分子生物学、生物化学、荧光活化细胞分类和主要体外用途(例如,克隆角质细胞)。

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Cite this Article

Morris, R. J., Readio, N., Boland, K., Johnson, K., Lad, S., Singh, A., Singh, A., Holtorf, S., Skaar, S. Isolation of Mouse Epidermal Keratinocytes and Their In Vitro Clonogenic Culture. J. Vis. Exp. (150), e58701, doi:10.3791/58701 (2019).

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