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Een Rat Methyl-Seq Platform om epigenetische veranderingen Associated with Stress Exposure te identificeren
JoVE Journal
Biochemie
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JoVE Journal Biochemie
A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure
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Een Rat Methyl-Seq Platform om epigenetische veranderingen Associated with Stress Exposure te identificeren

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09:06 min

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October 24, 2018

DOI:

10.3791/58617-v

DOI:
10.3791/58617-v

•

09:06 min
October 24, 2018

•

1 Views
, , , , , , ,
1Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, 2Department of Medicine,Johns Hopkins School of Medicine, 3Department of Mental Health,Johns Hopkins School of Public Health

Summary

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We beschrijven hier, het protocol en de uitvoering van Methyl-Seq, een epigenomic-platform, met behulp van een rat-model om epigenetische veranderingen die zijn gekoppeld aan chronische stress blootstelling te identificeren. Resultaten tonen aan dat de rat Methyl-Seq platform is voor het opsporen van methylation van verschillen die uit stress blootstelling bij ratten voortvloeien van.

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Cite this Article

Carey, J. L., Cox, O. H., Seifuddin, F., Marque, L., Tamashiro, K. L., Zandi, P. P., Wand, G. S., Lee, R. S. A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure. J. Vis. Exp. (140), e58617, doi:10.3791/58617 (2018).

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