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大鼠甲基 Seq 平台识别与应力接触相关的表观变化

JoVE Journal
Biochemie

This content is Free Access.

JoVE Journal Biochemie

A Rat Methyl-Seq Platform to Identify Epigenetic Changes Associated with Stress Exposure

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大鼠甲基 Seq 平台识别与应力接触相关的表观变化

10,381 Views

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09:06 min

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October 24, 2018

DOI:

10.3791/58617-v

DOI:

10.3791/58617-v

•

09:06 min

October 24, 2018

•

1 Views

Jenny L. Carey¹, Olivia H. Cox¹, Fayaz Seifuddin¹, Leonard Marque¹, Kellie L.K. Tamashiro¹, Peter P. Zandi^1,3, Gary S. Wand^1,2, Richard S. Lee¹

¹Departments of Psychiatry and Behavioral Sciences,Johns Hopkins School of Medicine, ²Department of Medicine,Johns Hopkins School of Medicine, ³Department of Mental Health,Johns Hopkins School of Public Health

Summary

在这里, 我们描述了同道平台甲基 Seq 的协议和实现, 使用老鼠模型来识别与慢性应力暴露相关的表观变化。结果表明, 大鼠甲基序列平台能够检测大鼠应力暴露引起的甲基化差异。

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