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如何定量光活化荧光蛋白在大容量和活细胞中的分数
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Biochemie
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JoVE Journal Biochemie
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
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如何定量光活化荧光蛋白在大容量和活细胞中的分数

6,496 Views

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11:03 min

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January 07, 2019

DOI:

10.3791/58588-v

DOI:
10.3791/58588-v

•

11:03 min
January 07, 2019

•

1 Views
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1Gynecologic Oncology Division,Stanford University School of Medicine

Summary

Automatically generated

在这里, 我们提出了一个协议, 涉及基因耦合光谱不同的光可激活和荧光蛋白。这些荧光蛋白嵌合体允许定量的 pa-fp 分数是光活化的荧光,即光活化效率。该协议揭示了不同的光活化模式产生不同的光活化效率。

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Cite this Article

Chen, V., Renz, M. How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells. J. Vis. Exp. (143), e58588, doi:10.3791/58588 (2019).

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