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1Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics,University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics,University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute,University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine
Kapitel
- 00:04Titel
- 00:36Cell Seeding in 24-well Plate
- 02:54Live Cell Analysis
- 03:51Analysis Parameter Setup
- 05:41Analysis and Data Processing
- 07:06Results: Measuring LDL Cholesterol Uptake in Various Cells Using Live Cell Imaging
- 08:05Conclusion
This protocol provides an efficient approach to measuring LDL cholesterol uptake with real time influx rates using a live cell imaging system in various cell types. This technique provides a platform to screen the pharmacological activity of compounds affecting LDL influx while monitoring for cell morphology and hence potential cytotoxicity.
