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1Department of Child Health,University of Arizona College of Medicine, 2Department of Biological Sciences,University of Bath, 3BARROW Neurological Institute at Phoenix Children’s Hospital, 4Phoenix Veteran Affairs Healthcare System, 5Department of Basic Medical Sciences,University of Arizona College of Medicine
Kapitel
- 00:04Titel
- 00:44Cryoprotection of Perfused Brains and Brain Freezing
- 03:18Preparing the Megabrain for Sectioning on the Cryostat
- 04:14Cryosectioning the Megabrain
- 05:24Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
- 05:54Conclusion
Qui, presentiamo un protocollo per congelare e sezione di tessuto cerebrale da più animali come alternativa ad alta produttività per singoli cervelli di elaborazione. Questo riduce la variabilità di colorazione durante immunohistochemistry e imaging e tempo cryosectioning.
