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Covalente immobilisatie van eiwitten voor de enkel molecuul Force spectroscopie

JoVE Journal
Biochemie

This content is Free Access.

JoVE Journal Biochemie

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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Covalente immobilisatie van eiwitten voor de enkel molecuul Force spectroscopie

10,574 Views

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11:13 min

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August 20, 2018

DOI:

10.3791/58167-v

DOI:

10.3791/58167-v

•

11:13 min

August 20, 2018

•

2 Views

Tanja D. Becke^2,3, Stefan Ness, Stefanie Sudhop³, Hermann E. Gaub, Markus Hilleringmann, Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Tags

Covalent Immobilization Protein Single Molecule Force Spectroscopy Atomic Force Microscope Cantilever Probe Biomolecule Immobilization Silanization EDC NHS Phosphate-buffered Saline

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