Journal
/
Biotechnik
/
Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells

80,402 Views

•

09:18 min

•

September 07, 2018

DOI:

10.3791/58154-v

DOI:
10.3791/58154-v

•

09:18 min
September 07, 2018

•

4 Views
, , , , , , ,
1European Molecular Biology Laboratory, 2Department of Infectious Diseases, Molecular Virology,Heidelberg University, 3Central Facility for Electron Microscopy,Ulm University, 4Heidelberg Partner Site,German Center for Infection Research

Tags

Correlative Light Electron Microscopy (CLEM)Viral ProteinCellular MembranesBio-replicationHigh Pressure FreezingCryo-immobilizationElectron MicroscopyLight MicroscopyFluorescent ProteinsVirus-infected CellsTransfected CellsUltrastructure Analysis

Read Article

Cite this Article

Santarella-Mellwig, R., Haselmann, U., Schieber, N. L., Walther, P., Schwab, Y., Antony, C., Bartenschlager, R., Romero-Brey, I. Correlative Light Electron Microscopy (CLEM) for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells. J. Vis. Exp. (139), e58154, doi:10.3791/58154 (2018).

Download .ris file

Copy

Share Video

.

Copy