/

Immunologie und Infektion

/

全血流式细胞术分析人单核细胞亚群的表征

JoVE Journal
Immunologie und Infektion

This content is Free Access.

JoVE Journal Immunologie und Infektion

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Please note that all translations are automatically generated. Click here for the English version.

全血流式细胞术分析人单核细胞亚群的表征

56,510 Views

•

09:12 min

•

October 17, 2018

DOI:

10.3791/57941-v

DOI:

10.3791/57941-v

•

09:12 min

October 17, 2018

•

37 Views

Rekha Marimuthu^1,2, Habib Francis^1,2, Suat Dervish³, Stephen C.H. Li⁴, Heather Medbury*^1,2, Helen Williams*^1,2

¹Department of Surgery, Vascular Biology Research Centre,Westmead Hospital, ²Westmead Clinical School, Department of Surgery,The University of Sydney, ³Westmead Research Hub,Westmead Institute for Medical Research, ⁴Institute for Clinical Pathology and Medical Research,Westmead Hospital

Summary

在这里, 我们提出了一个通过全血流式细胞仪表征单核细胞子集的协议。这包括概述如何门的子集和评估他们的表面标记的表达, 并提供了一个例子, 评估 M1 (炎症) 和 M2 标记 (抗炎) 的表达。

Explore More Videos

Monocyte Subsets Flow Cytometry Whole Blood Analysis CD14 CD16 HLA-DR M1 And M2 Markers Gating Red Blood Cell Lysis White Blood Cell Fixation Sample Preparation Flow Cytometry Setup Quality Control

Read Article