Journal
/
/
用于膜蛋白结构分析和从头建模的小角中子散射实验中的对比匹配洗涤剂
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Contrast-Matching Detergent in Small-Angle Neutron Scattering Experiments for Membrane Protein Structural Analysis and Ab Initio Modeling
DOI:

10:27 min

October 21, 2018

, , , , ,

Kapitel

  • 00:04Titel
  • 00:54Determine Neutron Contrast Match Points and Necessary Contrast for Protein Measurement
  • 02:05Express and Purify the Membrane Protein of Interest
  • 03:25Collect SANS Data and Reduce SANS Data from 2D Image to 1D Plot
  • 04:48Analyze Data for Structural Parameters of the Scattering Particle
  • 07:06Create Ab Initio Models from the SANS Data
  • 08:17Results: Visualization of the Experimental SANS Envelope and Overlay with High-resolution Model using PyMOL
  • 09:40Conclusion

Summary

Automatische Übersetzung

该协议演示了如何获得一个低分辨率的从头模型和洗涤剂-溶解膜蛋白在溶液中使用小角中子散射与洗涤剂的对比匹配的结构细节。

Verwandte Videos

Read Article