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Mundtlige bakterieinfektion og kaste i Drosophila melanogaster
JoVE Journal
Biologie
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JoVE Journal Biologie
Oral Bacterial Infection and Shedding in Drosophila melanogaster

Mundtlige bakterieinfektion og kaste i Drosophila melanogaster

DOI:
10.3791/57676-v

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09:32 min

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May 31, 2018

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, , , ,
1Institute of Evolutionary Biology, School of Biological Sciences,University of Edinburgh, 2IGDR – CNRS UMR 6290, 3Centre for Immunity, Infection and Evolution,University of Edinburgh

Kapitel

  • 00:05Titel
  • 00:46Maintaining and Preparing the Flies
  • 02:52Bacterial Culture
  • 04:15Orally Infecting Flies
  • 06:18Measuring Bacterial Load and Bacterial Shedding
  • 07:18Results: P. aeruginosa and P. entomophila Infections
  • 08:43Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Denne protokol beskriver metoder til mundtligt udsætte og inficere frugtflue Drosophila melanogaster med bakterielle patogener, og til at måle antallet af smitsomme bakterier skur efter tarm infektion. Vi yderligere at beskrive virkningen af immun mutanter på flyve overlevelse efter mundtlig bakteriel infektion.

Tags

Oral Bacterial InfectionDrosophila MelanogasterGut ImmunocompetenceHost DefensePathogenMicrobiology ProtocolsLewis MediumPopulation CageApple AgarPBSP. EntomophilaP. AeruginosaLB Broth

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