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利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
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利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维

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11:22 min

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June 27, 2018

DOI:

10.3791/57506-v

DOI:
10.3791/57506-v

•

11:22 min
June 27, 2018

•

3 Views
, , ,
1Laboratory of Molecular and Chemical Biology of Neurodegeneration,Ecole Polytechnique Fédérale de Lausanne (EPFL)

Summary

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在这里, 我们提出了一个健壮和优化的协议, 以生产毫克数量的本地, 无标签单体和纤维的 exon1 的杭丁顿蛋白蛋白 (Httex1) 的基础上的瞬态融合的小泛素相关修饰剂 (相扑)。

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Cite this Article

Reif, A., Chiki, A., Ricci, J., Lashuel, H. A. Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy. J. Vis. Exp. (136), e57506, doi:10.3791/57506 (2018).

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