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Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
DOI:

11:04 min

June 23, 2018

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Kapitel

  • 00:05Titel
  • 01:10S. cerevisiae Cultures and Protein Expression
  • 03:16Cell Staining
  • 04:29Flow Cytometry Analysis
  • 09:05Results: Protein Inclusions Play a Protective Role Against Oxidative Stress
  • 10:38Conclusion

Summary

Automatische Übersetzung

Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.

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