Journal
/
Biotechnik
/
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

7,886 Views

•

09:33 min

•

May 03, 2018

DOI:

10.3791/57440-v

DOI:
10.3791/57440-v

•

09:33 min
May 03, 2018

•

1 Views
, , , ,
1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute,University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute,University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute,University of Calgary

Summary

Automatically generated

Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.

Explore More Videos

Automated Slide ScanningWidefield High-content Analysis SystemProtein QuantificationFluorescent MicroscopyImage-based FocusingAutomated Segmentation Workflow

Read Article

Cite this Article

Poon, C. C., Ebacher, V., Liu, K., Yong, V. W., Kelly, J. J. P. Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System. J. Vis. Exp. (135), e57440, doi:10.3791/57440 (2018).

Download .ris file

Copy

Share Video

.

Copy