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Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System

7,919 Views

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09:33 min

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May 03, 2018

DOI:

10.3791/57440-v

DOI:
10.3791/57440-v

•

09:33 min
May 03, 2018

•

1 Views
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1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute,University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute,University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute,University of Calgary

Summary

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Here we describe a protocol for the automated segmentation of fluorescently labeled tissues on slides using a widefield high-content analysis system (WHCAS). This protocol has wide-ranging applications in any field which involves the quantitation of fluorescent markers in biological tissues including the biological sciences, medical engineering, and health sciences.

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Cite this Article

Poon, C. C., Ebacher, V., Liu, K., Yong, V. W., Kelly, J. J. P. Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System. J. Vis. Exp. (135), e57440, doi:10.3791/57440 (2018).

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