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JoVE Journal Chemistry
The MPLEx Protocol for Multi-omic Analyses of Soil Samples
 

The MPLEx Protocol for Multi-omic Analyses of Soil Samples

Article DOI: 10.3791/57343-v • 10:12 min • May 30th, 2018
May 30th, 2018 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Biological Sciences Division, Pacific Northwest National Laboratory
* These authors contributed equally

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Summary
Automatic Translation

A protocol is presented for simultaneously extracting metabolites, proteins, and lipids from a single soil sample, allowing reduced sample preparation times and enabling multi-omic mass spectrometry analyses of samples with limited quantities.

Tags

MPLEx Protocol Multi-omic Analyses Soil Samples Proteomics Metabolomics Lipidomics Environmental Perturbations Molecular Changes Analytes Sample Preparation Methanol/chloroform-compatible Tubes PTFE Tubes Chloroform-washed Stainless Steel Beads Ultrapure Water Ice Bucket Fume Hood Chloroform To Methanol Ratio Cell Wall Breakdown Enzymatic Activities Tube Vortex Attachments Negative 80 Degree Celsius Freezer
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