Journal
/
Biochemie
/
Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

7,461 Views

•

10:22 min

•

April 03, 2018

DOI:

10.3791/57279-v

DOI:
10.3791/57279-v

•

10:22 min
April 03, 2018

•

1 Views
, , ,
1Escuela Nacional de Ciencias Biológicas,Instituto Politécnico Nacional, 2Facultad de Medicina,Universidad Nacional Autónoma de México, 3Instituto de Bioquímica Médica Leopoldo de Meis,Universidade Federal do Rio de Janeiro

Summary

Automatically generated

Here, we describe a protocol to obtain the lipid droplet index (LD index) to study the dynamics of triacylglycerols in cells cultured in high-throughput experiments. The LD index assay is an easy and reliable method that uses BODIPY 493/503. This assay does not need dispendious lipid extraction or microscopy analysis.

Explore More Videos

Lipid Droplet IndexLipid DropletsLiquid Fluorescence RecoveryUstilago MaydisNutrient ConditionsBO-DP 493-503Quenching SolutionCell CultureOptical DensityCentrifugationFixationLipid Field

Read Article

Cite this Article

Romero-Aguilar, L., Montero-Lomeli, M., Pardo, J. P., Guerra- Sánchez, G. Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis. J. Vis. Exp. (134), e57279, doi:10.3791/57279 (2018).

Download .ris file

Copy

Share Video

.

Copy