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To-foton Intravital billeddannelse af leukocytter under immunrespons i LPS-behandlede mus lever
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

To-foton Intravital billeddannelse af leukocytter under immunrespons i LPS-behandlede mus lever

DOI:
10.3791/57191-v

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07:06 min

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February 06, 2018

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, , ,
1Department of Anatomy,Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science,Yonsei University College of Medicine, 3Department of Surgery,Yonsei University College of Medicine

Kapitel

  • 00:05Titel
  • 00:28Surgical Process
  • 03:06Intravital Imaging of the Liver with Two-photon MIcroscopy
  • 04:51Results: Two-photon Intravital Imaging in LPS-treated Mouse Liver
  • 06:03Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Vi etablerede en roman kirurgisk protokol for to-foton billeddannelse af levende mus lever med minimal invasion. Med denne teknik identificeret vi den detaljerede struktur i leveren under LPS-induceret endotoxemia. Vi forventer, at denne metode kan bruges til at bestemme effektiviteten af forskellige reagenser behandling af hepatisk leukocyt migration.

Tags

Two-photon Intravital ImagingLeukocytesImmune ResponseLipopolysaccharideMouse LiverSeptic ShockLiver SurgeryAnesthesiaTexas Red DextranPovidone IodineAbdominal CavityLiver ExposureIntravital ImagingLaser SettingsWater Immersion Objective LensTemperature Control

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