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Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Kapitel
- 00:04Titel
- 02:01Incubation of Carcinogen and Drugs with DNA in the Presence of Enzymatic Systems
- 03:04Isolation of the DNA from the Incubate
- 04:3032P-postlabeling for Detection of DNA Adduct Formation
- 05:04TLC Separation of Adducted Deoxyoligonucleotides
- 05:43Results: Study of the Potency of Metabolically Active Ellipticine Drug in Generating Covalent DNA Adducts
- 07:33Conclusion
Evaluating the potency of environmental chemicals and drugs, to be enzymatically bioactivated to intermediates generating covalent DNA adducts, is an important field in the development of cancer and its treatment. Methods are described for compound activation to form DNA adducts, as well as techniques for their detection and quantification.
