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针对天然产物的单克隆抗体的产生
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Generation of Monoclonal Antibodies Against Natural Products
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针对天然产物的单克隆抗体的产生

10,655 Views

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12:15 min

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April 06, 2019

DOI:

10.3791/57116-v

DOI:
10.3791/57116-v

•

12:15 min
April 06, 2019

•

1 Views
, , , , , ,
1School of Life Science,Beijing University of Chinese Medicine, 2Third Affiliated Hospital,Beijing University of Chinese Medicine, 3School of Basic Medical Sciences,Beijing University of Chinese Medicine, 4School of Chinese Materia Medica,Beijing University of Chinese Medicine, 5Center of Scientific Experiment,Beijing University of Chinese Medicine

Summary

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本文提供了一个详细的方案, 用于制备和评价用于各种免疫检测的天然产品单克隆抗体。该程序包括免疫接种、细胞融合、用于阳性克隆筛选的间接竞争性 ELISA 和单克隆杂交瘤制剂。还提供了使用 maldi TOF-MS 和 ELISA 分析进行抗体表征的规范。

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Zhang, Y., Cao, P., Lu, F., Yan, X., Jiang, B., Cheng, J., Qu, H. Generation of Monoclonal Antibodies Against Natural Products. J. Vis. Exp. (146), e57116, doi:10.3791/57116 (2019).

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