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Developmental Biology
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JoVE Journal Developmental Biology
Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model
 

Two-step Approach to Explore Early- and Late-stages of Organ Formation in the Avian Model: The Thymus and Parathyroid Glands Organogenesis Paradigm

Article DOI: 10.3791/57114-v • 13:43 min • June 17th, 2018
June 17th, 2018 •
1, 1
1Instituto de Histologia e Biologia do Desenvolvimento, Faculdade de Medicina, Universidade de Lisboa

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Summary
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This article provides an updated approach to the classical quail-chicken chimera system to study organ formation, by combining novel in vitro and in ovo experimental procedures.

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Two-step Approach Explore Early-stages Late-stages Organ Formation Avian Model Thymus Parathyroid Glands Organogenesis Paradigm In Vitro Manipulation Embryonic Tissues In Ovo Grafting Techniques Organotypic System Embryonic Territories Molecular Markers Experimental Manipulation Developmental Stages Spatial Modifications Tissue Interactions Cultured Tissues Chorioallantoic Membrane Chicken Embryo Vascular Supplier Nutrients Gas Exchanges In Ovo Development Fully Formed Organs Pharmacological Agents Signaling Pathways
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