JoVE
JoVE
Ressourcen für Lehrende
Forschung
Verhalten
Biochemie
Biologie
Biotechnik
Krebsforschung
Chemie
Entwicklungsbiologie
Ingenieurwesen
Umwelt
Genetik
Immunologie und Infektion
Medizin
Neurowissenschaften
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Lehre
Biologie
Chemie
Clinical
Ingenieurwesen
Umweltwissenschaften
Pharmacology
Physik
Psychologie
Statistik
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Autoren
Bibliothekare
Gymnasien
Über uns
Sign-In
Anmelden
Kontakt
Forschung
JoVE Journal
JoVE Encyclopedia of Experiments
Lehre
JoVE Core
JoVE Science Education
JoVE Lab Manual
Gymnasien
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Forschung
Verhalten
Biochemie
Biotechnik
Biologie
Krebsforschung
Chemie
Entwicklungsbiologie
Ingenieurwesen
Umwelt
Genetik
Immunologie und Infektion
Medizin
Neurowissenschaften
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Lehre
Biologie
Chemie
Clinical
Ingenieurwesen
Umweltwissenschaften
Pharmacology
Physik
Psychologie
Statistik
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Ihrem Kurs zugeordnete Videos
Teacher Resources
Get in Touch
Instant Trial
Log In
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Entwicklungsbiologie
/
使用磁纳米粒子转导病毒载体进行冷冻切片的原小鼠肠道器官的遗传工程
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.
Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal
Entwicklungsbiologie
Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning
Please note that all translations are automatically generated.
Click here for the English version.
使用磁纳米粒子转导病毒载体进行冷冻切片的原小鼠肠道器官的遗传工程
DOI:
10.3791/57040-v
•
07:37 min
•
May 10, 2019
•
Lingling Xian
,
Lionel Chia
5
,
Dan Georgess
,
Li Luo
,
Shuai Shuai
,
Andrew J. Ewald
4
,
Linda M.S. Resar
4,5,6
1
Department of Medicine, Division of Hematology
,
Johns Hopkins University School of Medicine
,
2
Department of Natural Sciences, School of Arts & Sciences
,
Lebanese American University
,
3
Department of Cell Biology
,
Johns Hopkins University School of Medicine
,
4
Department of Oncology
,
Johns Hopkins University School of Medicine
,
5
Department of Pathology
,
Johns Hopkins University School of Medicine
,
6
Institute for Cell Engineering
,
Johns Hopkins University School of Medicine
Kapitel
00:04
Titel
01:29
Genetic Engineering of Organoids by Viral Transduction
02:26
Seeding of Infected Organoid Fragments
03:44
Organoid Cryosection in Basement Membrane Matrix
05:32
Results: Efficient Viral Transduction of Intestinal Organoids
06:21
Conclusion
Summary
Automatische Übersetzung
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatische Übersetzung
我们描述了分步说明:1) 使用磁性纳米粒子对慢透或抗逆转录病毒转导进行高效工程肠道有机体,以及 2) 从工程有机物生成冷冻部分。这种方法提供了一个强大的工具,有效地改变基因表达在有机体的研究下游效应。
Tags
Genetic Engineering
Mouse Intestinal Organoids
Magnetic Nanoparticle Transduction
Viral Vectors
Frozen Sectioning
Organoid Cultures
Genetic Manipulation
Intestinal Epithelium
Developmental Processes
Pathologic Processes
Transduction
Basement Matrix
2D Cell Culture
Article
Embed
ZUR WIEDERGABELISTE HINZUFÜGEN
Usage Statistics
Verwandte Videos
使用非整合附加型质粒从冷冻血沉棕黄层诱导多能干细胞的产生
从鼠耳和尾组织生成主成纤维细胞培养
心肌细胞DNA的合成和倍性的同时评价:一个方法来帮助心肌细胞再生和营业额的量化
使用主神经球文化研究纤毛
免疫荧光标记的微管和中心蛋白在
体内
肠道组织和 3D
体外
肠道 Organoids
人肠道 Organoids 上皮屏障通透性的实时测量
小鼠肝外胆管有机体的产生
多细胞人类原发性子宫内膜器官的生成
使用纸屑小鼠研究矿化组织的克隆遗传追踪
使用胎儿肠道有机体在体外重述吸吮到发胶过渡
Read Article