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Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation
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Genetik
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JoVE Journal Genetik
Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

11,891 Views

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09:05 min

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December 25, 2017

DOI:

10.3791/56990-v

DOI:
10.3791/56990-v

•

09:05 min
December 25, 2017

•

2 Views
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1Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology,University of California, Riverside, 2Section of Cell and Developmental Biology, Division of Biological Sciences,University of California, San Diego

Summary

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Microinjection of Nasonia vitripennis embryos is an essential method for generating heritable genome modifications. Described here is a detailed procedure for microinjection and transplantation of Nasonia vitripennis embryos, which will greatly facilitate future genome manipulation in this organism.

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Li, M., Bui, M., Akbari, O. S. Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation. J. Vis. Exp. (130), e56990, doi:10.3791/56990 (2017).

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